Reporter

Part:BBa_I12019:Design

Designed by: Hans   Group: Antiquity   (2004-07-22)


Test Construct of BBa_I12007 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The test construct was built using BioBrick parts that were available and working. The premise behind this test construct is that the absence of p22 cII will yield a constant promotion of LacI, which will inhibit the expression of lambda cI. However, once IPTG is added, the IPTG will bind to the LacI, hence freeing the promoters of the QPI (Quad Part Inverter) and promoting production of lambda cI. Lambda cI will bind to the operators of BBa_I12007, thus promoting production of Yellow Fluorescent Protein


Source

References